Coding
Part:BBa_K2557001:Design
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-09)
Bxb1 recombinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 774
Illegal BamHI site found at 215
Illegal BamHI site found at 531
Illegal BamHI site found at 1239
Illegal BamHI site found at 1530 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 516
Illegal AgeI site found at 6 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 236
Design Notes
Since we used animal cells as the chassis organism, the coding sequence was humanized.
Source
We found the coding sequence of Bxb1 on NCBI and, after humanization, sent it to Kingsray for synthesis.