Coding

Part:BBa_K2557001:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


Bxb1 recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 774
    Illegal BamHI site found at 215
    Illegal BamHI site found at 531
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 1530
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 516
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 236


Design Notes

Since we used animal cells as the chassis organism, the coding sequence was humanized.


Source

We found the coding sequence of Bxb1 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References